Bright and Specific IL-17A Antibody From BioLegend

November 29, 2023

NA
Charite Medical University Berlin
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Brilliant Violet 711™ anti-human IL-17A Antibody

Catalog Number:

512327

Clone Number:

BL168

Functional characterization of T cell. Human PBMCs were stimulated with PMA/ionomycin for 5 hours in the presence of BFA. Cells were then surface stained with CD3/CD4/CD8, fixed, permeabilized, and then stained with IL-17A.

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

PBMC

Primary Incubation

PMA/Ionomycin for 5 hours

Blocking Agent

Human TruStain FcX™ (Fc Receptor Blocking Solution)

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Fortessa X20

Results Summary

Great IL-17A staining in PMA-Ionomycin stimulated CD4+ (CD8-) lymphocyte. 1:200 is an ideal concentration to separate between positive and negative population.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Bright and specific staining.

The Bad

N/A

The Bottom Line

Great staining of IL-17A.

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