BD Biosciences
BD OptiBuild™ BUV395 Rat Anti-Mouse I-A/I-E
743876
2G9
Info:Supplier Page
When used for flow cytometry on iMC38 tumor-infiltrating immune cells, there will be some background, but the staining on positive population is very strong
Flow Cytometry
Tumor-infiltrating immune cells from MC38 subcutaneous tumor
1/200 in PBS
Fc Block
None
BD LSR Fortessa II
Mouse tumor-infiltrating immune cells from MC38 subcutaneous tumors stained with this antibody show a very strong staining for MHCII
N/A
Very strong staining
Some background is present
Overall, staining for this antibody is very strong. Background is present and can be decreased with performing antibody titration.