A Good PE Anti-Mouse TCR γ/δ Antibody From BioLegend

December 05, 2023

Medicine
Weill Cornell Medicine
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

BioLegend

Product Name:

PE anti-mouse TCR γ/δ Antibody

Catalog Number:

118108

Clone Number:

GL3

When used for flow cytometry of intestinal lymphocytes, the detection of TCR γ/δ positive cells is good and allow separation from conventional TCR α/β T cells.

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

Mouse caecum cells

Primary Incubation

1/200 in PBS

Blocking Agent

Fc Block

Secondary Incubation

None

Tertiary Incubation

None

Detection

BD LSR Fortessa II

Results Summary

Mouse intestinal lymphocytes immune cells stained with this antibody show a clear population of TCR γ/δ+ T cells. I rate the staining as good to separation TCR γ/δ from TCR a/ß T cells.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Good and specific staining.

The Bad

The shift is decent could be stronger to facilitate separation from a/ß T cells.

The Bottom Line

Good staining to separate TCR γ/δ T cells from TCR a/ß T cells. Titration is recommended to improve the staining shift at its maximum.

Share your experience with other scientists. Write a Review! »

Join the discussion