Good Anti-Mouse CD127 Antibody From Biolegend

Medicine
Weill Cornell Medicine
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PE/Cyanine7 anti-mouse CD127 (IL-7Rα) Antibody

Catalog Number:

135014

Clone Number:

A7R34

Good staining of CD127 positive populations in mouse intestinal innate lymphoid cells. Great for detecting memory T cells and innate lymphoid cells

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

Mouse colonic cells

Primary Incubation

1/200

Blocking Agent

Fc Block

Secondary Incubation

None

Tertiary Incubation

None

Detection

BD LSR Fortessa II

Results Summary

Single cell suspension of mouse immune cells from intestinal tissues stained with this antibody shows a good shift separation allowing to detect lineage negative, CD127 positive innate lymphoid cells.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Good and specific staining.

The Bad

A little of background.

The Bottom Line

Good staining of CD127 in mouse intestinal immune cells. Great for detecting memory T cells and innate lymphoid cells.

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