BioLegend's Anti-Mouse CD127 Antibody Works Decently

November 10, 2023

WCM
Postdoctoral Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PE/Cyanine5 anti-mouse CD127 (IL-7Rα) Antibody

Catalog Number:

135016

Clone Number:

This antibody delivers solid results on LPMCs, however, some compensation difficulties are in line with fluorochrome.

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

LPMC

Primary Incubation

1:200 for 20min

Blocking Agent

Fc block

Secondary Incubation

None

Tertiary Incubation

None

Detection

FACS

Results Summary

This antibody delivers solid results on LPMCs, however some compensation difficulties in line with fluorochrome. Relatively bright staining compared to other fluorochromes I tested.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Bright staining.

The Bad

Compensation is difficult.

The Bottom Line

Great CD127 signal in convenient fluorochrome with difficult compensation.

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