Invitrogen
NuPAGE™ 4 to 12%, Bis-Tris Gel
NP0323BOX
Info:Supplier Page
To study the differential expression levels of various proteins involved in cellular signaling like c-Myc, mTOR, ERK, and p38 during viral infection, I have used these precast gels to resolve the cell lysate samples and further do the Western Blotting.
Protein electrophoresis
Cell lysate
Peel of the tap from the bottom of the gel before running.
Good separation of various proteins detected when stained with Coomassie blue stain. Also, clear bands of protein of interest were visualized after Western Blotting.
N/A
Saves lots of time compared to the conventional method.
None.
To get faster and reproducible results, I prefer these gels for my experiment.