Ideal Transfection Reagent From Thermo Fisher

November 08, 2023

UNC Chapel Hill

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher

Product Name:

Invitrogen™ Lipofectamine™ RNAiMAX Transfection Reagent

Catalog Number:

13778030

Lipofectamine RNAiMAX Transfection Reagent provides good transfection efficiencies on multiple cell types for siRNA-mediated gene knockdown experiments. Lipofectamine RNAiMAX is a proprietary RNAi-specific cationic lipid formulation designed specifically for the delivery of siRNA and miRNA into all cell types.

Experimental Design and Results Summary

Application

Transfection

Starting Material

Cultured mammalian cells

Tips

Optimize the amount based on cell line

Results Summary

I get a good transfection efficiency requiring lower RNAi concentrations, leading to more effective gene knockdown with minimal nonspecific effects. It is easy optimization due to minimal cytotoxicity across a 10-fold concentration range of transfection reagent.

DOI or PMID #

N/A

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Summary

The Good

Works well with all cell lines.

The Bad

Maybe a little expensive.

The Bottom Line

I mix Lipofectamine RNAiMAX Transfection Reagent with siRNA, add to the cells, incubate, and measure gene knockdown. The simplicity and speed combined with high transfection efficiency makes RNAiMAX Transfection Reagent ideal for high-throughput siRNA transfections. Transfection conditions are optimized easily and they have a good handbook that gives a good range.

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