Superb Antifungal Reagent for Media From MilliporeSigma

October 24, 2023

UNC Chapel Hill

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Company:

MilliporeSigma

Product Name:

Amphotericin B solution

Catalog Number:

A2942

Amphotericin B is a polyene antifungal antibiotic from Streptomyces sp. that is clinically used to treat systemic fungal infections. It has a high affinity for sterols, especially ergosterols, of both fungal and bacterial cell membranes. Normal usage in the maintenance of antifungal cell cultures is 2.5 mg/L with penicillin and streptomycin used in the medium.

Experimental Design and Results Summary

Application

Cell culture

Starting Material

Cultured mammalian cells

Tips

I used the recommended dilution.

Results Summary

Amphotericin B is most effective against fungi and yeast. It forms transmembrane channels by binding to sterols associated with membranes, thereby leading to the leakage of small molecules. Amphotericin B elicits toxicity despite being an effective antibiotic for treating mycoses. I use it when I have fungal contamination.

DOI or PMID #

N/A

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Summary

The Good

Works well with all cell lines.

The Bad

Can't think of anything bad.

The Bottom Line

This product is supplied as 250 μg/mL Amphotericin B in deionized water. It is a solution containing Amphotericin B, and the following components aimed to improve its solubility; sodium deoxycholate, sodium chloride and sodium phosphate.

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