Amicon Ultra 2 mL From MilliporeSigma for Volumes Less Than 5 mL

University of Texas Medical Branch at Galveston
OBGYN
Graduate Student

Overall

Performance

Ease-of-Optimization

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Company:

MilliporeSigma

Product Name:

Amicon Ultra 2mL

Catalog Number:

UFC201024

Our volume outputs for some experiments are less than 3 mL. Therefore, for concentrating proteins or filtering out big molecules, we use an Amicon tube. This size is convenient for our applications.

Experimental Design and Results Summary

Application

Exosome isolation

Tips

Need to invert the column to a collection tube.

DOI or PMID #

N/A

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Summary

The Good

Less than 5 mL volume, just 15 min spin time.

The Bad

Inversion of column may lead to sample loss.

The Bottom Line

A short 15 min spin time at 3000 g allows for efficient filtration of microvesicles out of our culture media. This tube is very convenient for smaller volumes instead of using the classic 50 mL Amicon.

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