Bio-Rad
iScript™ cDNA Synthesis Kit
1708890
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I have used an iScript cDNA synthesis kit to study the differential gene expression after viral infection in primary human epithelial cells. This cDNA synthesis was used at the first step of real-time qPCR to convert RNA into cDNA and after that cDNA was used as a template for RT-qPCR.
Real time-qPCR
RNA isolated from infected and non-infected human primary cells
Even diluted RNA can also be used in 20 ul reaction.
Low input RNA 100 ng and high input RNA 1 ug both efficiently converted to cDNA in 20 ul reaction volume.
N/A
Easy, precise and fast protocol to use this kit for cDNA synthesis.
None.
Highly recommended.