Dynabeads From Gibco™ Works as it is Intended to but Requires Optimization

September 11, 2023

Immunology
George Washington University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Gibco™

Product Name:

Dynabeads™ Human T-Activator CD3/CD28 for T Cell Expansion and Activation

Catalog Number:

11131D

Clone Number:

N/A

I used CD3/CD28 coupled Dynabeads to activate and expand my primary T cells isolated from peripheral blood mononuclear cells. While they work well and the instructions to use them are clear, it does require some optimization as you can under or over-activate your T cells. I would suggest testing different ratios of the Dynabeads to cells to see what works best for T cell expansion without impacting function (i.e. exhaustion, viability).

Experimental Design and Results Summary

Applications

T cell activation

Sample

Human primary T cells

Primary Incubation

Wash Dynabeads with buffer and incubate for five minutes; place on magnet to remove supernatant for at least one minute before adding cells

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

These Dynabeads helped to activate and expand my primary T cells from PBMCs in order to use them for downstream lentiviral applications. The activation was sufficient but I did experience some issues with expansion that I suspect were due to over-activation. Further optimiziation of using the Dynabeads with an appropriate bead-to-cell ratio would be needed to maximize results.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Works well at stimulating pan T cells activation and expansion.

The Bad

Expensive, more cumbersome having to wash and remove magnetic beads, and requires optimization of bead to cell ratio.

The Bottom Line

Expensive, likely requires optimization but is overall an effective reagent for T cell activation and expansion.

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