BioLegend's BV421 Anti-Mouse/Rat XCR1 Antibody Works Excellently

September 17, 2023

Department of Fundamental Oncology
University of Lausanne
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

BV421 anti-mouse/rat XCR1 Antibody

Catalog Number:

148216

Clone Number:

ZET

To titrate the antibody, 2 x 10^6 splenocytes from C57BL/6 mice were stained with PE anti-CD8a Ab and BV421 anti-XCR1 Ab in the presence of Zombie NIR in 100 µL PBS at 4C for 30 min.

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

Splenocytes from C57BL/6 mouse

Primary Incubation

See description

Blocking Agent

N/A

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

BD LSRFortessa

Results Summary

To titrate the antibody, 2 x 10^6 splenocytes from C57BL/6 mouse were stained with: FITC anti-CD11c Ab, PE anti-CD8a Ab and BV421 anti-XCR1 Ab in the presence of Zombie NIR in 100 μL PBS at 4C for 30 min.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Great resolution.

The Bad

N/A

The Bottom Line

According to the titration, 1:100 dilution has the best SI.

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