Excellent PE-Dazzle 594 Human CD186 Antibody From BioLegend

August 29, 2023

Microbiology and Immunology
Columbia University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

BioLegend

Product Name:

PE/Dazzle™ 594 anti-human CD186 (CXCR6) Antibody

Catalog Number:

356016

Clone Number:

N/A

I used it for phenotyping human PBMC by flow cytometry to study immune memory in inflammatory disease. 5 lase Cytek Aurora was used and it works well.

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

Human PBMC

Primary Incubation

1:50

Blocking Agent

Fc block

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

N/A

Results Summary

I tittered the antibody and ended up using 1:50 dilution with 5% FBS 2mM EDTA in PBS. Incubation for 30min RT works great and shows positive population well.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Works well.

The Bad

None.

The Bottom Line

Antibody works very well, would buy again.

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