High Efficiency Transfection Reagent From Promega

August 27, 2023

Immunology
George Washington University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

Promega

Product Name:

ViaFect™ Transfection Reagent

Catalog Number:

E4981

Clone Number:

N/A

I used ViaFect as my transfection reagent for HEK293T to generate lentivirus. I had a very smooth experience with using ViaFect and achieved high-quality lentivirus generation. The added convenience of not needing to remove ViaFect following transfection was a plus, and I would use ViaFect for further transfections in other cell lines.

Experimental Design and Results Summary

Applications

Cell transfection

Sample

HEK293T

Primary Incubation

Complex with DNA for 20 minutes at RT before adding dropwise to cells.

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

None

Results Summary

ViaFect enabled me to successfully transfect HEK293T with my lentiviral vectors and generate lentivirus for my downstream transduction experiment. I experienced no issues with cytotoxicity or transfection efficiency using this reagent and have consistently gotten reproducible results.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Minimal cytotoxicity and highly efficient transfection.

The Bad

None.

The Bottom Line

If you are curious about using the reagent, Promega is always great with providing a sample for you to test. However, I would say that ViaFect is an excellent transfection reagent that would likely work for a variety of cell lines without major optimization.

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