PE Anti-Human CD127 Flow Cytometry Antibody From Miltenyi Biotec

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Quality of Results

Ease-of-Optimization

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Company:

Miltenyi Biotec

Product Name:

CD127 Antibody, anti-human, PE

Catalog Number:

130-113-410

Clone Number:

MB15-18C9

I wanted to stain the expression of CD127 on the surface of peripheral NK cells. Therefore, I stained human PBMCs in serial dilutions from 1:25, 1:50, 1:100, 1:200, 1:400, and 1:800 respectively from left to right as seen in the image attached.

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

Human PBMC

Primary Incubation

30 minutes at room temperature.

Blocking Agent

PBS/BSA

Secondary Incubation

None

Tertiary Incubation

None

Detection

yg-586/15

Results Summary

This antibody is very bright and I really like it. It performs at all serial dilutions and CD127 often has a continuous expression.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Very bright.

The Bad

Nothing.

The Bottom Line

I would continue to use this antibody.

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