Easy to Use Human B-Cell Isolation Kit (Negative selection) From Stemcell Technologies

August 10, 2023

Immunology and Microbiology
The Scripps Research Institute
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Stemcell Technologies

Product Name:

EasySep™ Human B Cell Enrichment Kit

Catalog Number:

19054

Clone Number:

N/A

Info: Supplier Page 

We are a genome engineering lab working towards engineering B cells to create next-generation vaccines. The first step of every genome engineering experiment is to enrich human B cells from a leukopak. The EasySep™ Human B Cell Enrichment Kit provides a simple and fast solution for our application.

Experimental Design and Results Summary

Applications

Cell Isolation/Separation

Sample

Human leukopak

Primary Incubation

Re-suspension of cells at 50 million per mL density, Enrichment cocktail 50 μL/mL, Incubate for 10 min at room temperature.

Blocking Agent

N/A

Secondary Incubation

Magnetic particles at 75 μL/mL, Incubate for 5 min at room temperature.

Tertiary Incubation

N/A

Detection

N/A

Results Summary

Using the EasySep Human B cell enrichment kit we obtained B cells with 98-99% purity. The results were consistent among multiple donor samples. It was an easy and quick procedure (within 30 minutes) to enrich B cells from human leukopaks.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Simple, easy and quick protocol.

The Bad

Requires additional component, the magnet which is not included in the kit and might increase the total cost of the experiment. For processing more than one samples at a time, the EasyEights magnet needs to be purchased.

The Bottom Line

This kit is useful to obtain pure B cells from human leukopak or PBMCs. The magnet need for separation needs to be purchased separately. Highly recommended for quick processing of the samples.

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