Great Staining for Human CD19 With Antibody From BioLegend

August 04, 2023

Wayne State University

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Pacific Blue™ Anti-Human CD19

Catalog Number:

982404

Clone Number:

HIB19

Staining for human CD19 is strong and specific. Easy to separate B cells by flow cytometry. Not much background.

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

Human tonsil

Primary Incubation

1:200 for 15 min at 4°C

Blocking Agent

Fc block

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Fortessa LSRII

Results Summary

Single cell suspension from human tonsils stained with this antibody show specific staining of B cells. The population is clearly separated from remaining cells making B cell analysis easy.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Great staining for B cells.

The Bad

N/A

The Bottom Line

For analyzing human B cells, this antibody works very well. It is not particularly bright so may require a slightly higher concentration.

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