Measuring Na+ Concentration Using ING-2 AM From Ion Biosciences

July 31, 2023

Stanford University
Biochemistry
Postdoctoral Researcher

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Company:

ION Biosciences

Product Name:

ING-2 AM

Catalog Number:

2011C

Info: Supplier Page 

I am interested in measuring sodium ion levels in cells because sodium plays a crucial role in various cellular processes, including maintaining cell volume, regulating membrane potential, and influencing cell signaling. Understanding intracellular sodium dynamics can provide insights into cellular homeostasis and signaling pathways, contributing to a better understanding of cellular physiology. I employed fluorescence-based sodium indicator dye (ING-2 AM) and microscopy to measure sodium ion levels in cells, enabling real-time visualization and quantification of intracellular sodium concentrations.

Experimental Design and Results Summary

Application

Sodium concentration measurements

Starting Material

N/A

Tips

Use pluronic F127 for better dye penetration.

Results Summary

The cell images clearly indicate an increase in intracellular sodium levels, as evidenced by the higher fluorescence signal intensity observed. Imaging was performed by exciting at 488 nm and collecting emission 530/30 nm.

DOI or PMID #

N/A

Image Gallery

Summary

The Good

Easy staining procedure, quick, and can be used for both Immunofluorescence and Flow cytometry.

The Bad

Nothing.

The Bottom Line

Using ING-2 AM allows for the fluorescence-based measurement of intracellular sodium levels, enabling real-time visualization and quantification of this essential ion in cells.

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