X-tremeGENE™ 9 DNA Transfection Reagent for Transfecting Mammalian Cells

July 30, 2023

Stanford University
Biochemistry
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Roche

Product Name:

X-tremeGENE™ 9 DNA Transfection Reagent

Catalog Number:

6365787001

In my research, I aim to study protein localization and interactions by transiently transfecting cells using transfection. Transfection is a powerful method that allows for the efficient delivery of genetic material into cells, enabling the short-term expression of target proteins. By transiently transfecting cells, I can observe the dynamic behavior of proteins within the cellular environment without altering the cell's genome permanently. This approach will provide valuable insights into the spatial distribution and potential interactions of the proteins under investigation, contributing to a better understanding of cellular processes and protein functions.

Experimental Design and Results Summary

Application

Transfection of cells

Starting Material

Cells on tissue-culture plates.

Tips

Avoid adding the reagent directly on plastic (microfuge tube) surface.

Results Summary

X-tremeGENE™ 9 DNA Transfection Reagent proved highly efficient in facilitating successful transfection of NIH3T3 cells, a challenging cell type that is difficult to transfect using other methods like PEI. The results demonstrated successful protein expression, as evidenced by a blot analysis following transient transfection of the protein of interest in NIH/3T3 cells.

DOI or PMID #

N/A

Image Gallery

Summary

The Good

X-tremeGENE™ 9 DNA Transfection Reagent is a highly efficient and reliable tool for delivering DNA into a wide range of cell types, enabling successful transfection with excellent gene expression and minimal cytotoxicity.

The Bad

None.

The Bottom Line

X-tremeGENE™ 9 DNA Transfection Reagent offers efficient and low-toxicity DNA delivery for successful transfection across diverse cell types.

Join the discussion