Thermo Scientific™
B-PER™ Complete Bacterial Protein Extraction Reagent
89821
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As a researcher working with purified proteins from bacteria, the B-PER™ Complete Bacterial Protein Extraction Reagent has become an indispensable tool in my research. Its role in lysing bacterial cells efficiently is crucial, as it enables to extract total proteins with high yield and minimal degradation. It enables rapid (15 min incubation) and effective disruption of bacterial cell walls (due to added lysozyme), ensuring maximum protein yield with minimal degradation. This extraction reagent is invaluable for various downstream applications, such as Western blotting, enzyme assays, and protein analysis, facilitating accurate and reproducible results in bacterial protein research.
Lysis of bacteria
Bacterial pellet (fresh or frozen)
Follow manufacturer's protocol for adding 1 mM EDTA/protease inhibitors/reducing agents.
I have utilized the B-PER™ Complete Bacterial Protein Extraction Reagent for lysing cells and subsequent protein purification,. The reagent's rapid action and non-requirement for sonication have made the cell lysis process significantly more efficient and convenient. Moreover, its ability to yield a high quantity of proteins has been instrumental in obtaining ample purified protein samples for my research, saving valuable time and ensuring successful downstream applications.
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Rapid and ready to use.
None.
The B-PER™ Complete Bacterial Protein Extraction Reagent is a rapid and efficient solution for cell lysis and protein purification, offering high protein yields without the need for sonication.