Amicon® Ultra-15 Centrifugal Filters for Concentrating Proteins From Millipore

Stanford University
Biochemistry
Postdoctoral Researcher

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Company:

Millipore

Product Name:

Amicon® Ultra-15 Centrifugal Filter Unit

Catalog Number:

UFC903024

Info: Supplier Page 

Amicon® Ultra-15 Centrifugal Filters are essential for concentrating proteins in biological research and biotechnological applications. Their efficient centrifugation process allows for rapid concentration, enabling researchers to save time and achieve higher protein yields. These filters are particularly valuable when working with dilute protein samples, as they efficiently remove excess buffer and small molecules, leading to increased protein concentration and purity.

Experimental Design and Results Summary

Application

Concentrating proteins/exchanging buffer.

Starting Material

Protein in buffer

Tips

Follow manufacturer's protocol for centrifugal force and duration.

Results Summary

In the image of the Amicon® Ultra-15 Centrifugal Filter, the separation of the mCherry-tagged protein with a molecular weight of ~100 kDa is evident. The filtrate's colorless appearance indicates successful filtration, effectively removing proteins below 30 kDa. This outcome demonstrates the filter's efficiency in concentrating and purifying the target protein, making it a valuable tool for protein research and downstream applications.

DOI or PMID #

N/A

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Summary

The Good

Rapid method for concentrating proteins and exchanging buffers.

The Bad

None.

The Bottom Line

Efficiently concentrate and purify proteins, ensuring high yields and exceptional purity.

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