Thermo Fisher Scientific
Power SYBR Green Cells-to-CT Kit
4402955
Info: Supplier Page
Hedgehog signaling plays a critical role in embryonic development. It also controls adult tissue homeostasis and deregulation of this pathway results in basal cell carcinoma and medulloblastoma. A unique feature of this pathway is that it requires a tiny antenna-like organelle called the primary cilium for signal transduction. My goal is to understand the molecular mechanism of hedgehog signal transduction at the primary cilium. This involves detecting protein-protein interactions of the pathway components, analysis of localization patterns during signal transduction, and transcriptional output measurements. The specific goal of this experiment is to determine the responsiveness of NIH-3T3 cells to various sonic hedgehog ligand concentrations.
qRT-PCR
NIH-3T3 cells
Manufacturer's instructions were strictly followed in this experiment.
20,000 cells/well were seeded in a 96 well plate (3 wells for each ligand concentration). 48 hrs later, treatment was done in the presence of low serum for 24 hrs.
N/A
Saves time.
None.
This kit is really good for large sample sets (like 96-well plates) and easy to use.