Electroporation Device for mESCs

July 21, 2023

Stanford University
Biochemistry
Senior Research Scientist

Overall

Performance

Ease-of-Optimization

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Company:

Bulldog Bio

Product Name:

NEPA21 Electro-Kinetic Transfection System

Catalog Number:

NEPA21

We routinely perform protein over-expression experiments in various cell lines to understand their role in signaling pathways. One of the major challenges is to achieve high transfection efficiencies. NEPA21 solves this major hurdle.

Experimental Design and Results Summary

Application

Electroporation of cell lines

Tips

N/A

DOI or PMID #

N/A

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Summary

The Good

Electroporation medium is Opti-MEM and can try various parameters.

The Bad

None.

The Bottom Line

Voltage and pulse duration in this experiment were tried and >70% transfection efficiency was achieved. The vendor was very helpful during the troubleshooting process to achieve best results.

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