Very Clear Staining With Cyclin E1 Rabbit mAb From Cell Signaling

July 28, 2023

Discovery
Tav
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Cyclin E1 (D7T3U) Rabbit mAb #20808

Catalog Number:

20808S

Clone Number:

D7T3U

We were testing the effects of a knockout/knockdown of an E3-ubiquitin ligase known to interact with CCNE1.

Experimental Design and Results Summary

Applications

Western blot

Sample

SKOV3

Primary Incubation

1:1000, 4°C Overnight

Blocking Agent

Blocking Buffer

Secondary Incubation

1hr RT

Tertiary Incubation

N/A

Detection

IRDye 800CW

Results Summary

This antibody was able to detect differences in CCNE1 levels with treatment, decreasing the amount of this specific E3-ubiquitin ligase increases the amount of CCNE1 protein.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Worked great.

The Bad

No issues.

The Bottom Line

Antibody has a great signal when used at 1:1000 dilution.

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