Cell Signaling Technology
IKKβ (D30C6)
8943
N/A
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The enzyme IKK-β serves as a protein subunit of IκB kinase. It is a component of the cytokine-activated intracellular signaling pathway which in turn leads to triggering immune responses. Due to this activity by IKK causes activation of a NF-κB. This activated IKK-β then phosphorylates IκBα, which binds NF-κB to inhibit its function. Phosphorylated IκB will then degraded via the ubiquitination pathway. Due to this act the NFKB will entry into the nucleus of the cell where it activates other genes involved in inflammation and other immune responses. Here we checked the time course effect of IKKBeta phosphorylation by chemoattractant.
Western Blot
Mouse Lysate
1:1000 prepared in 1x TBST
5% Skim milk
1:4000 prepared in 1x TBST
None
ECL
We performed western blot and detect, analyzed the IKKβ expression. We found that there is a a time dependent increase of IKKβ expression by chemoattractant.
This antibody works great for western blot.
This antibody is very specific and gives good signal. Highly recommend.