Easy to Use SYBR Green Reagent by ELK Biotech

May 23, 2023

NTHU
IMCB
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

ELK Biotechnology

Product Name:

EnTurbo™ SYBR Green PCR SuperMix

Catalog Number:

EQ001

The reagent is used to detect for the presence of cDNA in our samples using qPCR. It is a premixed reagent that only requires the user to add their samples and primers to the well for detection.

Experimental Design and Results Summary

Application

qPCR

Starting Material

N/A

Tips

Have to adhere to the primer concentration provided by the manufacturer.

Results Summary

The reagent is less sensitive compared to other reagents that we have used as it requires a higher concentration of primers to be used. If a lower concentration of primer is used, there will be a high standard deviation among the technical triplicates.

DOI or PMID #

N/A

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Summary

The Good

Easy to use.

The Bad

Low sensitivity compared to other type of SYBR green reagents.

The Bottom Line

This reagent is not recommended due to its low sensitivity and requires a higher amount of primers added to each samples. Therefore, users can consider other options available in the market other than this.

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