Antibody From BD Biosciences Stains TNFa Very Well

Stanford

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Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

BD Horizon™ BV711 Rat Anti-Mouse TNF

Catalog Number:

563944

Clone Number:

MP6-XT22

Info: Supplier Page 

Browse Similar Products: TNF Flow Cytometry Antibody Products

Our lab works in the field of cancer, and we treated mice with immunotherapy. We wanted to assess how immunotherapy in tumor-bearing mice would affect immune cell cytokine production in the spleen, so we used the antibody to identify TNFa+ immune cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Single-cell suspension of mouse spleen.

Primary Incubation

1:100

Blocking Agent

FcBlock

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Cytek Aurora

Results Summary

We stained mouse spleen cells with the anti-TNFa antibody and found that it identifies TNFa-producing immune cells very well. The antibody was easy to use and the staining was very clear.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

The antibody works great for staining TNFa.

The Bad

None to mention.

The Bottom Line

The antibody identifies TNFa-producing immune cells very clearly.

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