Fairly Good CD127 Antibody for Flow Cytometry

April 27, 2023

UCSF
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Anti-mouse CD127 (IL-7Ra) in BV785

Catalog Number:

135037

Clone Number:

A7R34

Info:Supplier Page View Product Specs

Browse Similar Products: CD127 Antibodies

I use this antibody to detect CD127 positivity in murine lymphocytes via flow cytometry. It works fairly well as it has strong signal, but the separation between positive and negative populations is not super clear.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine lymphocytes

Primary Incubation

1:300, 4C for 20 min

Blocking Agent

Fc block

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometry

Results Summary

This product is helpful to identify CD127+ and CD127- subpopulations via spectral flow cytometry among murine lymphocytes.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Strong signal

The Bad

Hard to gate positive from negative subpopulation.

The Bottom Line

This antibody works fairly well but could be better for flow.

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