Good Antibody to Identify TCRgd+ Murine Lymphocytes

April 12, 2023

UCSF
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific

Product Name:

Anti-mouse TCRgd antibody in PerCP/eF710

Catalog Number:

46-5711-82

Clone Number:

GL3

This antibody is in my regular panel to identify murine lymphocyte subpopulations. This antibody has strong signal and fairly good separation.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine Lymphocytes

Primary Incubation

1:500, 4C for 20 minutes

Blocking Agent

Fc block

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometry

Results Summary

This product helps me distinguish TCRgd+ subpopulations via flow cytometry within murine lymph nodes. This antibody has good signal and fairly good separation.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Strong signal, fairly good separation.

The Bad

None

The Bottom Line

I recommend this antibody to identify TCRgd+ and TCRgd- populations in murine lymph nodes by flow cytometry.

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