Great Resolution with BioLegend's BV650 Anti-Mouse CD11c Antibody

April 05, 2023

Department of Fundamental Oncology
University of Lausanne
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Brilliant Violet 421™ anti-mouse CD186 (CXCR6) Antibody

Catalog Number:

151109

Clone Number:

SA051D1

Info:Supplier Page View Product Specs

Browse Similar Products: CD186 Antibodies

To titrate the antibody, 2 x 10^6 splenocytes from C57BL/6 mouse were stained with: PerCP-Cy5.5 anti-CD4 Ab and BV421 anti-CXCR6 Ab in the presence of Zombie NIR in 100 μL PBS at 4C for 30 min.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Splenocytes from C57BL/6 mouse

Primary Incubation

See description

Blocking Agent

N/A

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

BD LSRFortessa

Results Summary

To titrate the antibody, 2 x 10^6 splenocytes from C57BL/6 mouse were stained with: PerCP-Cy5.5 anti-CD4 Ab and BV421 anti-CXCR6 Ab in the presence of Zombie NIR in 100 μL PBS at 4C for 30 min.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Great resolution

The Bad

N/A

The Bottom Line

According to the titration, 1:400 dilution has the best SI.

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