Good Signal for Selecting Positive Cells

March 07, 2023

UNIVERSITY OF ALABAMA AT BIRMINGHAM
Pharmacology
Research Scientist

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Quality of Results

Ease-of-Optimization

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Company:

Integrated DNA Technologies

Product Name:

Alt-R™ S.p. Cas9-GFP V3,

Catalog Number:

10008100

Cas9-GFP was used for form RNP complex to knockout gene in vitro. The results showed that the GFP signal is relative strong and easy to select GFP positive cell to enhance RNP-targeted cell collection.

Experimental Design and Results Summary

Application

cas9 targeted cell selection

Starting Material

Transfected cell

Tips

Sorting cell after 24 hours

Results Summary

Cas9-GFP was used for form RNP complex to knockout gene in vitro. The results showed that the GFP signal is relative strong and easy to select GFP positive cell to enhance RNP-targeted cell collection.

DOI or PMID #

N/A

Image Gallery

Summary

The Good

Good signal for cell sorting.

The Bad

N/A

The Bottom Line

Cas9-GFP formed RNP complex facilitates positive cell selection.

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