GM130 as a Golgi Marker for Immunofluorescence in Cultured Hippocampal Neurons

March 02, 2023

Vascular Dysfunction
University of Heidelberg
Postdoctoral Researcher

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Company:

BD Biosciences

Product Name:

Anti-GM130 antibody

Catalog Number:

#610822

Clone Number:

N/A

Info:Supplier Page View Product Specs

Browse Similar Products: GM130 Antibodies

GM130 antibody specifically labels the golgi apparatus in the cell. Cultured hippocampal neurons were stained with GM130 antibody to determine the position of golgi apparatus in neuronal soma.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Hippocampal neuron culture at DIV 14

Primary Incubation

1:200, overnight at 4 degrees

Blocking Agent

10% NDS and 0.3 TritonX 100 in PBS

Secondary Incubation

1:500, 2 hours at RT

Tertiary Incubation

None

Detection

Immunofluorescence

Results Summary

GM130 antibody specifically labelled the cis-golgi apparatus in the cultured neurons. this allowed us to determine the localisation of our protein of interest.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Specific antibody for labelling cis-golgi region of the golgi apparatus.

The Bad

None

The Bottom Line

Highly specific antibody, great signal - recommended.

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