Good Transfectant Reagent from Roche

February 10, 2023

University of Cote d'Azur (UCA)
LP2M
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Roche

Product Name:

X-tremeGENE™ HP DNA Transfection Reagent

Catalog Number:

6366244001

We work on cells lines and we perform routine transfections on different clones of HEK293 line.This reactive performs very well in all of them and it is easier to use compare to other suppliers.

Experimental Design and Results Summary

Application

Cells transfection

Starting Material

Immortalized cell lines

Tips

The plasmid must be very clean (isopropanol precipitation os not recommended).

Results Summary

User-friendly reactive for routine transfection, it performs excellently in different clone of HEK cells and do not require specific buffer or protocol.

DOI or PMID #

N/A

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Summary

The Good

Ona step protocol to prepare the liposome.

The Bad

N/A

The Bottom Line

Excellent transfection reagent, it doesn't require specific solution for the formation of the complexes, transfection can be performed in complete cell medium without toxicity even if the medium with complexes is not removed up to 72 hours.

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