Excellent Kit for Direct IgG2a Coupling

University of Cote d'Azur (UCA)
LP2M
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Invitrogen

Product Name:

Zenon™ Mouse IgG2a Labeling Kits

Catalog Number:

Z25102

Working on cell surface proteins that are very instable and fast internalized, we needed a fast and reliable kit to directly couple mouse IgG2a antibody and reduce the incubation time of staining steps. This kit allows a single and rapid incubation with the already FITCH couple antibody, avoiding the loss of fluorescence due to complexes internalisation.

Experimental Design and Results Summary

Application

Cell surface staining/FACS

Starting Material

Cells

Tips

Follow the protocol.

Results Summary

Very useful and easy kit to direct couple any mouse IgG2a antibody with Alexa488, reducing incubation steps and increasing the signal for cell surface protein that are rapidly degraded.

DOI or PMID #

N/A

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Summary

The Good

Very easy and fast (10mins protocol).

The Bad

N/A

The Bottom Line

Recommended kit for the IgG2a direct coupling, 10 minutes protocol and very strong signal.

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