Cell Signaling Technology
SAPK/JNK Antibody
9252
N/A
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The antibody was diluted with TBST 1:1000 (v/v) and the membrane was incubated overnight at 4 °C. Fibroblast (MRC-5, human cells line) were induced with tgf-b with starvation medium (0.5% FBS) for 24 hours and harvested with ripa buffer (100 ul).
Western Blot
Fibroblast
1:1000, 4 °C, overnight
BSA 5% TBST
1:10000
Chemiluminescence
The antibody was diluted with TBST 1:1000 (v/v) and the membrane was incubated overnight at 4 °C. Fibroblast (MRC-5, human cells line) were induced with tgf-b with starvation medium (0.5% FBS) for 24 hours and harvested with RIPA buffer (100 ul).
Band is clear.
Recommended.