S6 Ribosomal Protein Antibody Works Well for Western Blot

January 24, 2023

Houston Methodist Research Institute

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Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

S6 Ribosomal Protein (5G10) Rabbit mAb

Catalog Number:

2217L

Clone Number:

N/A

The cells (macrophage) were seeded for 24 hours in 10% FBS RPMI. The following day were stimulated with tgf-b (5 ng/ml) for 24 hours in starvation medium (0.5% FBS). Cells were harvested after 24 h with ripa buffer (100 ul)

Experimental Design and Results Summary

Applications

Western Blot

Sample

Macrophage (MH-S)

Primary Incubation

1:1000, 4 °C, overnight

Blocking Agent

BSA 5% TBST

Secondary Incubation

1:10000

Tertiary Incubation

N/A

Detection

Chemiluminescence

Results Summary

The cells (macrophage) were seeded for 24 hours in 10% FBS RPMI. The following day were stimulated with tgf-b (5 ng/ml) for 24 hours in starvation medium (0.5% FBS). Cells were harvested after 24 h with ripa buffer (100 ul)

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Band is clear.

The Bad

N/A

The Bottom Line

Recommended.

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