Phospho-SAPK/JNK Antibody for Western Blot

January 11, 2023

Houston Methodist Research Institute

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Cell Signaling Technology

Product Name:

Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Mouse mAb

Catalog Number:

9255S

Clone Number:

N/A

I used this antibody diluted in TBST 1:1000 (v/v) and I incubate overnight at 4 °C. The cells (fibroblast) were induced with tgf-b for 24 hours and harvested with ripa buffer

Experimental Design and Results Summary

Applications

Western Blot

Sample

Fibroblast

Primary Incubation

1:1000, 4 °C, overnight

Blocking Agent

BSA 5% TBST

Secondary Incubation

1:10000

Tertiary Incubation

N/A

Detection

Chemiluminescence

Results Summary

I used this antibody diluted in TBST 1:1000 (v/v) and I incubate overnight at 4 °C. The cells (fibroblast) were induced with tgf-b for 24 hours and harvested with ripa buffer

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Band is clear.

The Bad

N/A

The Bottom Line

Really recommended.

Share your experience with other scientists. Write a Review! »

Join the discussion