Good Antibody to Detect SOD1

December 20, 2022

Pharmacology
PennState College of Medicine
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific

Product Name:

SOD1 Polyclonal Antibody

Catalog Number:

PA1-30195

Clone Number:

N/A

Info:Supplier Page View Product Specs

Browse Similar Products: SOD1 Antibodies

This antibody works really well at the suggested concentration and it's a good antibody to assess SOD1 level via western blot.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mammalian cell line whole cell lysate

Primary Incubation

1:1000, overnight incubation at 4 degree Celsius

Blocking Agent

5% BSA in TBST

Secondary Incubation

Goat anti-rabbit IgG HRP Ab, dilution (1:2500) in 5% BSA , 1hr at RT

Tertiary Incubation

N/A

Detection

ECL

Results Summary

Antibodies reacted very well, without many non-specific bands. We were able to detect the SOD1 level by western blotting.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Strong signal.

The Bad

Nonspecificity

The Bottom Line

Very good antibody to assess SOD1 level via western blotting.

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