Fast and Precise Isolation Kit

University of Cote d'Azur (UCA)
LP2M
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

StemCell Technologies

Product Name:

EasySep Mouse T Cell Isolation Kit

Catalog Number:

19851

Our study required ultra pure and untouched murin lymphocytes T and we previously used columns based purification. With this kit, we speed up the purification (negative selection) to 15-20 minutes from the collection of the spleen to the pure T cells. The kit is very simple to use and the results are impressive in matter of quality and quantity of obtained cells.

Experimental Design and Results Summary

Application

Primary cell isolation (T cells)

Starting Material

Spleen

Tips

Following the protocol is the only tip you need.

Results Summary

This kit helped us to drastically decrease the hands-on time in the T cell purification protocols with an amelioration of the yield.

DOI or PMID #

N/A

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Summary

The Good

Very rapid and easy to use, no columns needed.

The Bad

Eh, more expensive than other kits.

The Bottom Line

I suggest this kit if you are looking for an efficient and rapid way to isolate untouched T cells from mouse spleen or other sources (blood, ganglions). The manual is very clear and straight forward and there is no need of using disposable columns for every experiment, but you still have to equip yourself with the proper supermagnetic holder.

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