Cell Trace CFSE Cell Proliferation Kit

Houston Methodist

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Quality of Results

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Company:

Thermo Fisher Scientific

Product Name:

Cell trace CFSE cell proliferation kit

Catalog Number:

C34554

CFSE is a versatile tool for the fluorescent intracellular labeling of live cells. Labeled cells can be assayed using flow cytometry and fluorescent microscopy. The dye is long lasting and well retained within labeled cells. The provided CFSE is sufficient for ~1000 assays.

Experimental Design and Results Summary

Application

Cell viability

Starting Material

N/A

Tips

None - follow instructions

Results Summary

CFSE (Carboxyfluorescein succinimidyl ester) is a membrane-permeable fluorescent dye to track viable cells. CFSE is non-fluorescent until it enters viable cells, where it is hydrolyzed by cytoplasmic esterase enzymes to release fluorescent amine-reactive dyes.

DOI or PMID #

N/A

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Summary

The Good

Efficient and specific

The Bad

None, overall the only issues depend on the quality of sample.

The Bottom Line

Overall is good product, especially for flow cytometry application.

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