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FITC anti-human CD127 (IL-7Rα) Antibody
351311
A019D5
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I wanted to stain the expression of CD127 on the surface of peripheral T cells and CD56bright NK cells, so I therefore stained human PBMCs in serial dilutions from 1:25, 1:50, 1:100, 1:200, 1:400 and 1:800 respectively from left to right as seen in the image attached.
Flow Cytometry
Human PBMC
30 minutes at room temperature
PBS/BSA
None
b-530/30
This antibody worked quite well as the signal did not drop with higher dilutions. Therefore I probably will use at 1:800.
N/A
Consistently bright.
Nothing!
I will use this in a larger phenotyping, but not sorting panel.