LoxP GFP/RFP Color Switch Lentivirus for Cell Models

UNIVERSITY OF ALABAMA AT BIRMINGHAM

Overall

Quality of Results

Ease-of-Optimization

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Company:

GenTarget Inc

Product Name:

LoxP GFP/RFP ColorSwitch (Bsd) lentivirus

Catalog Number:

LVP460-Bsd-PBS

Info: Supplier Page 

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This is a pre-made lentivirus expressing the “LoxP-GFP-stop-LoxP-RFP-Stop” cassette. The solution contains a virus vector with 5×10^7 IFU/ml in PBS. 10 ul was used in HEK293 (1x10^6) for 24 hours. GFP was examined after 72 hours, and almost 98% of cells get infected.

Experimental Design and Results Summary

Application

Vector used to establish GFP-positive cell model

Starting Material

HEK293 cell

Tips

10 ul virus vector used in 10^6 cells

Results Summary

This is a pre-made lentivirus express the “LoxP-GFP-stop-LoxP-RFP-Stop” cassette. The solution contains a virus vector with 5×10^7 IFU/ml in PBS. 10 ul was used in HEK293 (1x10^6) for 24 hours. GFP was examined after 72 hours and almost 98% cells get infected.

DOI or PMID #

N/A

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Summary

The Good

Virus titer is pretty high enough.

The Bad

N/A

The Bottom Line

This virus vector is high concentrated and is easy used to establish cell model.

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