CD34 Staining for Mouse BM Cells

Advanced Diagnostics
UofAlberta
Postdoctoral Researcher

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Company:

BioLegend

Product Name:

PE anti-mouse CD34 Antibody

Catalog Number:

152203

Clone Number:

SA376A4

Info:Supplier Page View Product Specs

Browse Similar Products: CD34 Antibodies

This product was used to analyze the bone marrow cell composition in mice by flow cytometry. Not all anti-CD34 antibodies stain BM cells, while they label peripheral cells. This antibody stains both.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Bone marrow

Primary Incubation

0.2uL/50uL of FACS buffer; 30' at 4C (dark)

Blocking Agent

TruStain FcX™ (Biolegend)

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometry

Results Summary

Bone marrow cells were harvested by flushing C57BL/6 femurs with PBS. After hemolysis, cells were stained for flow cytometry. This antibody successfully labelled the CD34-positive population in the murine bone marrow.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Stains both peripheral and BM cells

The Bad

N/A

The Bottom Line

This is a good clone to label CD34-positive bone marrow cells in mice.

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