The Good
Use a small amount of protein to run the assay with more than one antibody.
The Bad
Requires a optimization for each one of the antibodies for each type of cell.
The Bottom Line
It is a great product for small amount of protein. After optimization, I needed only 0.2ug/ul of protein to run the assay, using a 1/50 primary antibody dilution. So far, I was able to optimize 3 antibodies running in the same capillary.