Great Anti-NFkB p50 Antibody

August 29, 2022

Surgery
University of Pittsburgh
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

BioLegend

Product Name:

anti-NFkB p50 antibody

Catalog Number:

616701

Clone Number:

4D1

Info:Supplier Page View Product Specs

Browse Similar Products: p50 Antibodies

BT20 cells were treated with 30 ng/ml TNFalpha for 30 minutes and NFkB p50 was examined by Western blot to check the response of NFkB canonical signaling pathway. The results showed that this pathway was activated after TNFalpha treatment.

Experimental Design and Results Summary

Applications

Western Blot

Sample

BT20

Primary Incubation

1/1000 dilution 4 degrees overnight

Blocking Agent

5% milk

Secondary Incubation

1:1000 dilution

Tertiary Incubation

Room temperature 1 hour

Detection

ECL

Results Summary

BT20 cells were treated with 30 ng/ml TNFalpha for 30 minutes and NFkB p50 was examined by Western blot to check the response of NFkB canonical signaling pathway. The results showed that this pathway was activated after TNFalpha treatment.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

The antibody works.

The Bad

N/A

The Bottom Line

The antibody works well.

Share your experience with other scientists. Write a Review! »

Join the discussion