Great PhosSTOP™ Buffer for Cell Lysis

Texas A&M University
Veterinary medicine
Rotating research student/Research technician

Overall

Quality of Results

Ease-of-Optimization

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Company:

Roche

Product Name:

PhosSTOP phosphatase inhibitor

Catalog Number:

04906837001

I love this product and use it all the time for my cell lysis. I have used this for western blot lysis and even for cell fractionation. It is easy to mix.

Experimental Design and Results Summary

Application

Cell lysis

Starting Material

N/A

Tips

I use the dilution as recommended.

Results Summary

I have used this with multiple applications. I usually mix it well before use. Once it is mixed well in the buffer, I incubate the solution for 30 minutes on ice with intermittent vortexing, then spin the mixture down.

DOI or PMID #

N/A

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Summary

The Good

Works well, good price.

The Bad

Can't think of anything bad.

The Bottom Line

I have used liquid inhibitors before but i like this more because I don't have to thaw anything before use. I just add this directly to the buffer and mix.

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