Great for Knockdowns

August 22, 2022

Texas A&M University
Veterinary medicine
Rotating research student/Research technician

Overall

Quality of Results

Ease-of-Optimization

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Company:

Invitrogen

Product Name:

Lipfectamine RNAi Max

Catalog Number:

13778-150

I use this for knocking down genes, and it has worked really well. The maximum knockdown I have gotten is 95%. The knockdown is not very stable, but it works well for my needs.

Experimental Design and Results Summary

Application

siRNA mediated knockdown (KD)

Starting Material

None

Tips

Change media the next day.

Results Summary

I have used this a lot and in some cells it works better than others. It is a little expensive but you can optimize the amount per reaction needed.

DOI or PMID #

N/A

Image Gallery

Summary

The Good

It works well on most cell lines.

The Bad

Expensive

The Bottom Line

I have used many transfection reagents and this one is my favorite by far. It is great and works on most of the cell lines. I also didn't have to optimize it a lot. The same protocol works for most of my cancer cell lines.

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