Clean Membrane for Clear Detection of Western Blotting

August 05, 2022

MD Anderson Cancer Center - University of Texas
Cancer System Imaging (Diagnostic Imaging Division)
Lab Technician

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Bio-Rad

Product Name:

Blotting Grade Blocker Non Fat Dry Milk

Catalog Number:

1706404XTU

I always use Blotting Grade Blocker Non-Fat Dry Milk (NFDM) as a blocking buffer for saturating excess protein-binding sites on membranes when doing Western blot, only when I don't use phosphor-specific antibodies. I usually use around 5% for most Western blots, and I always obtain clear and clean membranes.

Experimental Design and Results Summary

Application

Western Blot - Blotting Grade Blocker

Starting Material

Protein sample

Tips

I usually use 5% for most Western blots and this give me always better results.

Results Summary

I used Blotting Grade Blocker Non Fat Dry Milk (NFDM) of BIORAD as a blocking buffer for saturating excess protein-binding sites on membranes doing a Western blot (using a non-phosphor-specific antibody). I obtained a very clean membrane and a clear detection of the bands when imaging.

DOI or PMID #

N/A

Image Gallery

Summary

The Good

Clean membranes and clear bands detection.

The Bad

N/A

The Bottom Line

When doing Western blots with non-phosphor-specific antibodies, I usually prefer to use this NFDM as blotting grade blocker agent for saturating excess protein-binding sites. In particular, the BIORAD agent always gave me clean membrane and this mean clear detection of the protein bands.

Join the discussion