BD Biosciences
BD OptiBuild™ BUV805 Hamster Anti-Mouse CD3e
741928
500A2
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Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of B cells and antibody-secreting cells (ASC) in health and disease, in this experiment, we are looking for markers of PC. We wanted to have a good CD3 to exclude T cells from our analysis.
Flow Cytometry
Mouse splenocytes
Surface staining with CD3 BUV805 (1/100), 20 min at 4C
Fc block (2.4G2 clone)
N/A
Flow cytometer
We were looking for a good T cell marker in a not so common colour to exclude T cells from our analysis. This antibody stains T cells but is not bright enough.
It works.
Too expensive and not bright enough.
There are better alternatives in the market.