Good Anti-IL-8 Antibody [807] for Western Blot

July 22, 2022

Biology of Aging
MC
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Anti-IL-8 antibody [807]

Catalog Number:

ab18672

Clone Number:

807

Info:Supplier Page View Product Specs

Browse Similar Products: IL-8 Antibodies

This antibody was used to detect IL-8 levels from human cell lysates (25 ug/mL protein loading) through western blot analysis.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human cells

Primary Incubation

1:500, overnight@4ºC

Blocking Agent

5% BSA/TBS

Secondary Incubation

Anti-mouse IgG HRP (#HAF007, R&D systems); 1:5000

Tertiary Incubation

N/A

Detection

Chemiluminescent

Results Summary

The antibody can be reused more than 5x when kept at 4ºC in BSA/TBS. Bands (12 kDa) are strong at lower exposures.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Sensitive, robust, dilution in BSA/TBS can be reused.

The Bad

None

The Bottom Line

Very good antibody. Provides consistent results.

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